Investigating Microbial Eukaryotic Diversity from a Global Census: Insights from a Comparison of Pyrotag and Full-Length Sequences of 18S rRNA Genes

@article{Lie2014InvestigatingME,
  title={Investigating Microbial Eukaryotic Diversity from a Global Census: Insights from a Comparison of Pyrotag and Full-Length Sequences of 18S rRNA Genes},
  author={Alle A Y Lie and Zhenfeng Liu and Sarah K. Hu and A. Jones and Diane Y. Kim and P. Countway and L. Amaral-Zettler and S. Cary and E. Sherr and B. Sherr and R. Gast and D. Caron},
  journal={Applied and Environmental Microbiology},
  year={2014},
  volume={80},
  pages={4363 - 4373}
}
ABSTRACT Next-generation DNA sequencing (NGS) approaches are rapidly surpassing Sanger sequencing for characterizing the diversity of natural microbial communities. Despite this rapid transition, few comparisons exist between Sanger sequences and the generally much shorter reads of NGS. Operational taxonomic units (OTUs) derived from full-length (Sanger sequencing) and pyrotag (454 sequencing of the V9 hypervariable region) sequences of 18S rRNA genes from 10 global samples were analyzed in… Expand
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