Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine

  title={Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine},
  author={Oliver S. May and Peter T. Nguyen and Frances H. Arnold},
  journal={Nature Biotechnology},
Using directed evolution, we have improved the hydantoinase process for production of L-methionine (L-met) in Escherichia coli. This was accomplished by inverting the enantioselectivity and increasing the total activity of a key enzyme in a whole-cell catalyst. The selectivity of all known hydantoinases for D-5-(2-methylthioethyl)hydantoin (D-MTEH) over the L-enantiomer leads to the accumulation of intermediates and reduced productivity for the L-amino acid. We used random mutagenesis… 
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