Inverse Fusion PCR Cloning

Abstract

Inverse fusion PCR cloning (IFPC) is an easy, PCR based three-step cloning method that allows the seamless and directional insertion of PCR products into virtually all plasmids, this with a free choice of the insertion site. The PCR-derived inserts contain a vector-complementary 5'-end that allows a fusion with the vector by an overlap extension PCR, and… (More)
DOI: 10.1371/journal.pone.0035407

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Cite this paper

@inproceedings{Spiliotis2012InverseFP, title={Inverse Fusion PCR Cloning}, author={Markus Spiliotis}, booktitle={PloS one}, year={2012} }