Introns in protein‐coding genes in Archaea

  title={Introns in protein‐coding genes in Archaea},
  author={Yoh‐ichi Watanabe and Shin-Ichi Yokobori and T Inaba and Akihiko Yamagishi and Tairo Oshima and Yutaka Kawarabayasi and Hisasi Kikuchi and Kiyoshi Kita},
  journal={FEBS Letters},

Archaeal pre-mRNA splicing: a connection to hetero-oligomeric splicing endonuclease.


The novel tertiary structure of the splicing endonuclease is solved, and it is revealed that the lineage-specific insertion of amino acid residues in the end onuclease expands the recognition of the substrate precursor RNA.

Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea

In this study, CRISPRi was employed to repress expression of the splicing endonuclease in the archaeon Haloferax volcanii to identify all substrates of this enzyme and isolated and sequenced circular RNAs, which identified excised introns removed from tRNA and rRNA precursors as well as from the 5′ UTR of the gene HVO_1309.

Two reactions of Haloferax volcanii RNA splicing enzymes: joining of exons and circularization of introns.

The cells of Haloferax volcanii, a Euryarchaeote, are shown to contain circular RNAs formed by 3',5'-phosphodiester linkage between the two termini of the introns derived from their pre-tRNAs.

In silico screening of archaeal tRNA-encoding genes having multiple introns with bulge-helix-bulge splicing motifs.

By a comprehensive search for tRNA genes in 29 archaeal genomes using SPLITSX, 43 novel candidates that contain introns at noncanonical sites are found, including 15 that contained two introns and three that contained three introns within the respective putative t RNA genes.

Comparative Detection of Processed Small RNAs in Archaea

An analysis of the viability of using pattern-based and stochastic structural scanning algorithms for in silico studies of the occurrence of BHB motifs and comment on splicing motifs in other small RNAs, which mostly do not follow the pattern of bulge-helix-bulge motifs.

Transfer RNA genes in pieces

An overview of the various types of fragmented tRNA genes is provided and the hypothesis that the integration of mobile genetic elements—including viruses and plasmids—established such genes in pieces is examined.

Archaeal and eukaryotic homologs of Hfq

The archaeal domain of life is a missing link, and an opportunity, in Sm-based RNA biology, by leveraging the homology between these lineages to make phylogenetic inferences about Sm functions in archaea.

concept co cept Transfer RNA genes in pieces

An overview of the various types of fragmented tRNA genes is provided and the hypothesis that the integration of mobile genetic elements—including viruses and plasmids—established such genes in pieces is examined.



Ribosomal RNA introns in archaea and evidence for RNA conformational changes associated with splicing.

  • J. KjemsR. Garrett
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1991
The single 23S rRNA gene of the archaeon Staphylothermus marinus exhibits two introns which, at the RNA level, are located in highly conserved regions of domains IV and V, and parts of this structural feature that are essential for cleavage are defined using a phylogenetic sequence comparison approach.

RNA–protein interactions of an archaeal homotetrameric splicing endoribonuclease with an exceptional evolutionary history

The splicing endoribonuclease from Methanococcus jannaschii, a member of a recently defined family of enzymes involved in splicing of archaeal introns and eukaryotic nuclear tRNA introns, was

Splicing of Intron-containing tRNATrp by the ArchaeonHaloferax volcanii Occurs Independent of Mature tRNA Structure*

Results verify that the substrate recognition properties of the halobacterial endonuclease observed in vitro reflect the properties of this enzyme in vivo, namely that mature tRNA structure is not essential for recognition by the endonUClease.

Comparison of transfer RNA and ribosomal RNA intron splicing in the extreme thermophile and archaebacterium Desulfurococcus mobilis.

The structure of the exon-intron boundary was compared for an intron within 23S ribosomal RNA of Desulfurococcus mobilis and a newly discovered intron in tRNA(Met) from the same organism and suggests that intron excision occurs by the same mechanism.

Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria.

It is raised the possibility that pseudouridine formation in archaebacterial rRNA may be dependent on analogs of the eukaryotic box H/ACA snoRNPs, whose evolutionary origin may predate the split between Archaea and Eucarya.

The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus

The A. fulgidus genome encodes functionally uncharacterized yet conserved proteins, two-thirds of which are shared with M. jannaschii (428 ORFs), indicating substantial archaeal gene diversity.

An intron in the 23S ribosomal RNA gene of the archaebacterium Desulfurococcus mobilis

The first evidence for the occurrence of an intron within any prokaryotic ribosomal RNA was found within the 23S rRNA gene of the sulphur-dependent and anaerobic Thermoproteale Desulfurococcus mobilis, which was isolated from hot acidic springs in Iceland at temperatures up to 97°C.