Introns boost transgene expression in Drosophila melanogaster

@article{Duncker1997IntronsBT,
  title={Introns boost transgene expression in Drosophila melanogaster},
  author={Bernard P. Duncker and Peter L Davies and Virginia K. Walker},
  journal={Molecular and General Genetics MGG},
  year={1997},
  volume={254},
  pages={291-296}
}
Using Drosophila as a host, we have examined the effect that the presence of an intron has on the accumulation of a processed transgene mRNA. To provide a model system that was free from major position effects, two fish antifreeze protein (AFP) transgenes were arranged in divergent transcriptional orientation from a central Drosophila yolk protein promoter/enhancer region and introduced into the flies by P-element transformation and/or mobilization. In this way the organization of both the… CONTINUE READING

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