Intron-mediated enhancement of heterologous gene expression in maize

  title={Intron-mediated enhancement of heterologous gene expression in maize},
  author={Desmond Mascarenhas and Irvin J. Mettler and Dorothy A. Pierce and Heather W. Lowe},
  journal={Plant Molecular Biology},
Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the inclusion of maize alcohol dehydrogenase-1 introns 2 and 6 in the chimeric construct. This enhancement was seen when the intron was placed within the 5′ translated region but not… CONTINUE READING
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