Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.

@article{Rose2000IntronmediatedEO,
  title={Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.},
  author={Alan B Rose and Jason Beliakoff},
  journal={Plant physiology},
  year={2000},
  volume={122 2},
  pages={535-42}
}
Either of the first two introns of the Arabidopsis tryptophan pathway gene PAT1 elevates mRNA accumulation from a PAT1:beta-glucuronidase (GUS) fusion roughly 5-fold without affecting the rate of PAT1:GUS transcription. To further explore the mechanism of this intron-mediated enhancement of gene expression, we wanted to determine whether splicing or specific intron sequences were necessary. In-frame derivatives of PAT1 intron 1, whose splicing was prevented by a point mutation or large… CONTINUE READING
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Arabidopsis consensus intron sequences

  • AR Buchman, P Berg
  • Plant Mol Biol
  • 1996

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