Intron-mediated RNA interference and microRNA (miRNA).

@article{Lin2008IntronmediatedRI,
  title={Intron-mediated RNA interference and microRNA (miRNA).},
  author={Shi-Lung Lin and Ho-jin Kim and S Y Ying},
  journal={Frontiers in bioscience : a journal and virtual library},
  year={2008},
  volume={13},
  pages={
          2216-30
        }
}
MicroRNAs (miRNAs) are small single-stranded regulatory RNAs capable of interfering with messenger RNAs (mRNAs) through complete or partial complementarities. Partial complementarity gives miRNAs a flexibility which is useful for construction of new therapies against cancer polymorphisms and viral mutations. Varieties of miRNAs have been reported in diverse species; and they are believed to induce RNA interference (RNAi), a post-transcriptional gene silencing mechanism. Recently, many intronic… 

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References

SHOWING 1-10 OF 74 REFERENCES
Gene silencing in vitro and in vivo using intronic microRNAs.
TLDR
It is shown for the first time that intron-derived miRNA is not only able to induce RNAi in mammalian cells but also in fish, chicken embryos, and adult mice, demonstrating the evolutionary preservation of this gene regulation system in vivo.
MicroRNAs and Other Tiny Endogenous RNAs in C. elegans
MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
  • Yan Zeng, R. Yi, B. Cullen
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
TLDR
It is demonstrated that an endogenously encoded human miRNA is able to cleave an mRNA bearing fully complementary target sites, whereas an exogenously supplied siRNA can inhibit the expression of an RNA bearing partially complementary sequences without inducing detectable RNA cleavage.
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155
TLDR
New RNA polymerase II expression vectors for RNAi are developed, designated SIBR vectors, based upon the non-coding RNA BIC, and it is found that expression of a short region of the third exon of mouse BIC is sufficient to produce miR-155 in mammalian cells.
New microRNAs from mouse and human.
TLDR
31 novel miRNAs are described that were identified by cloning from mouse tissues and the human Saos-2 cell line and are important for assisting bioinformatics target mRNA identification and comprehensive genome annotation.
Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells
TLDR
A mammalian Pol III promoter system is described capable of expressing functional double-stranded siRNAs following transfection into human cells and achieving up to 4 logs of inhibition of expression from the HIV-1 DNA.
The nuclear RNase III Drosha initiates microRNA processing
TLDR
The two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation.
siRNA and miRNA: an insight into RISCs.
  • G. Tang
  • Biology
    Trends in biochemical sciences
  • 2005
Identification of mammalian microRNA host genes and transcription units.
TLDR
It is strongly suggested that miRNAs are transcribed in parallel with their host transcripts, and that the two different transcription classes of miRNAAs ('exonic' and 'intronic') identified here may require slightly different mechanisms of biogenesis.
A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution.
...
1
2
3
4
5
...