Intron-mediated RNA interference and microRNA (miRNA).
@article{Lin2008IntronmediatedRI,
title={Intron-mediated RNA interference and microRNA (miRNA).},
author={Shi-Lung Lin and Ho-jin Kim and S Y Ying},
journal={Frontiers in bioscience : a journal and virtual library},
year={2008},
volume={13},
pages={
2216-30
}
}MicroRNAs (miRNAs) are small single-stranded regulatory RNAs capable of interfering with messenger RNAs (mRNAs) through complete or partial complementarities. Partial complementarity gives miRNAs a flexibility which is useful for construction of new therapies against cancer polymorphisms and viral mutations. Varieties of miRNAs have been reported in diverse species; and they are believed to induce RNA interference (RNAi), a post-transcriptional gene silencing mechanism. Recently, many intronic…
60 Citations
Intron-mediated RNA interference, intronic microRNAs, and applications.
- BiologyMethods in molecular biology
- 2010
It is shown that, for the first time, intronic-derived miRNAs are able to induce RNA interference not only in human and mouse cell lines but also in zebrafish, chicken, and mouse, which demonstrates the evolutionary preservation of the intron-mediated gene silencing through miRNA functionality in cell and in vivo.
MicroRNA Protocols
- BiologyMethods in Molecular Biology
- 2013
The biogenesis and identi fi cation of miRNAs, potential applications, and future directions for research are presented in this chapter, hopefully providing a guideline for further miRNA and gene function studies.
MicroRNA: Biogenesis, Function and Role in Cancer
- BiologyCurrent genomics
- 2010
The P-body model outlines microRNA sorting and shuttling between specialized P- body compartments that house enzymes required for slicer –dependent and –independent silencing, addressing the reversibility of these silencing mechanisms.
A multiplexed miRNA and transgene expression platform for simultaneous repression and expression of protein coding sequences.
- BiologyMolecular bioSystems
- 2016
The current platform technology offers a miRNA/shRNA scaffold for the expression of combinations of native or synthetic intronic miRNAs as singletons or polycistrons for combinatorial multiplexed RNAi silencing or RNA-based gene therapy applications.
A novel class of endogenous shRNAs in human cells
- Biology
- 2008
Overall, this work reports the identification of 1258 distinct sRNAs derived from intronic regions of protein-coding genes in human with a new approach that appear to be similar to exogenous shRNAs in structure, have a broad distribution in the stem length, and function as microRNAs (miRNAs), small interfering RNAs (siRNA) and/or piwi-interacting RNAAs (piRNAs).
Exceptional stories of microRNAs
- BiologyExperimental biology and medicine
- 2013
Newly discovered information about the biogenesis and functions of miRNAs are described as well as different points of view about the miRNA system are presented.
MicroRNA Interference Technologies
- Biology
- 2009
The miRNA pathways are highly responsive to interventions of any kind, being excellent candidates for pharmacological manipulation, and new technologies for manipulating miRNAs are in need.
A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia.
- BiologyRNA
- 2011
In vivo and in vitro evidence suggested that the Giardia Dicer protein (GlDcr) is required for miR4 biogenesis, suggesting a canonical miRNA biogenesis pathway in Giardio, and a complex mechanism of miRNA regulation of VSP expression and the presence of a rich miRNAome in Giardy.
The art of microRNA: various strategies leading to gene silencing via an ancient pathway.
- BiologyBiochimica et biophysica acta
- 2008
Giardia lamblia regulates variant surface protein gene expression in A microRNA derived from an apparent canonical biogenesis pathway Material
- Biology
- 2011
In vivo and in vitro evidence suggested that the Giardia Dicer protein (GlDcr) is required for miR4 biogenesis, suggesting a canonical miRNA biogenesis pathway in Giardio, but derived from an unannotated open reading frame (ORF) rather than a snoRNA.
References
SHOWING 1-10 OF 74 REFERENCES
Gene silencing in vitro and in vivo using intronic microRNAs.
- BiologyMethods in molecular biology
- 2006
It is shown for the first time that intron-derived miRNA is not only able to induce RNAi in mammalian cells but also in fish, chicken embryos, and adult mice, demonstrating the evolutionary preservation of this gene regulation system in vivo.
MicroRNAs and Other Tiny Endogenous RNAs in C. elegans
- BiologyCurrent Biology
- 2003
MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 2003
It is demonstrated that an endogenously encoded human miRNA is able to cleave an mRNA bearing fully complementary target sites, whereas an exogenously supplied siRNA can inhibit the expression of an RNA bearing partially complementary sequences without inducing detectable RNA cleavage.
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155
- BiologyNucleic acids research
- 2006
New RNA polymerase II expression vectors for RNAi are developed, designated SIBR vectors, based upon the non-coding RNA BIC, and it is found that expression of a short region of the third exon of mouse BIC is sufficient to produce miR-155 in mammalian cells.
New microRNAs from mouse and human.
- BiologyRNA
- 2003
31 novel miRNAs are described that were identified by cloning from mouse tissues and the human Saos-2 cell line and are important for assisting bioinformatics target mRNA identification and comprehensive genome annotation.
Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells
- BiologyNature Biotechnology
- 2002
A mammalian Pol III promoter system is described capable of expressing functional double-stranded siRNAs following transfection into human cells and achieving up to 4 logs of inhibition of expression from the HIV-1 DNA.
The nuclear RNase III Drosha initiates microRNA processing
- BiologyNature
- 2003
The two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation.
Identification of mammalian microRNA host genes and transcription units.
- BiologyGenome research
- 2004
It is strongly suggested that miRNAs are transcribed in parallel with their host transcripts, and that the two different transcription classes of miRNAAs ('exonic' and 'intronic') identified here may require slightly different mechanisms of biogenesis.
A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution.
- BiologyBiochemical and biophysical research communications
- 2003






