Introduction to the synthesis and purification of oligonucleotides.


Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo- and oligoribonucleotides with a variety of labels, modified linkages, and nonstandard bases. Strategies are given for the maximization of… (More)
DOI: 10.1002/0471142700.nca03cs00


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@article{Ellington2001IntroductionTT, title={Introduction to the synthesis and purification of oligonucleotides.}, author={Andrew D. Ellington and Jane D. Pollard}, journal={Current protocols in nucleic acid chemistry}, year={2001}, volume={Appendix 3}, pages={Appendix 3C} }