Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker.

@article{Copass1997IntroductionOU,
  title={Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker.},
  author={Michael Copass and Guido Grandi and Rino Rappuoli},
  journal={Infection and immunity},
  year={1997},
  volume={65 5},
  pages={1949-52}
}
Research on Helicobacter pylori has been hindered by the lack of useful genetic tools. Using the sacB gene of Bacillus subtilis, we developed a sucrose-based counterselection system that allows introduction of unmarked mutations in H. pylori. A kan-sacB cassette, consisting of the sacB gene expressed from the H. pylori flagellin promoter and the kanamycin resistance module, was introduced by homologous recombination into a target H. pylori gene. The resultant strains were sucrose sensitive and… CONTINUE READING

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