Introduction of the carbohydrate-activated promoter PmalK for recombinant protein production

  title={Introduction of the carbohydrate-activated promoter PmalK for recombinant protein production},
  author={Maria Kristina Bostr{\"o}m and Gen Larsson},
  journal={Applied Microbiology and Biotechnology},
A production protocol for the use of the malK promoter was established. The protocol includes two phases: an initial fed-batch phase on glucose to reach a high cell density and a fed-batch phase on maltose for production of the desired recombinant protein. It is suggested that this cultivation scheme could be used for all promoters that are catabolite repressed by glucose and where growth and production need to be separated. The specific feature of this system is shown by its ability to control… CONTINUE READING
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