Introduction of site-specific mutations into the genome of influenza virus.

@article{Enami1990IntroductionOS,
  title={Introduction of site-specific mutations into the genome of influenza virus.},
  author={Masayoshi Enami and Willem Luytjes and Mark Leonia Krystal and P. Palese},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 10},
  pages={
          3802-5
        }
}
We succeeded in rescuing infectious influenza virus by transfecting cells with RNAs derived from specific recombinant DNAs. RNA corresponding to the neuraminidase (NA) gene of influenza A/WSN/33 (WSN) virus was transcribed in vitro from plasmid DNA and, following the addition of purified influenza virus RNA polymerase complex, was transfected into MDBK cells. Superinfection with helper virus lacking the WSN NA gene resulted in the release of virus containing the WSN NA gene. We then introduced… CONTINUE READING
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