Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse

@article{Williams1984IntroductionON,
  title={Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse},
  author={David A Williams and Ihor Lemischka and David G. Nathan and Richard C. Mulligan},
  journal={Nature},
  year={1984},
  volume={310},
  pages={476-480}
}
An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact… CONTINUE READING