Introduction of mRNA to plant protoplasts using polyethylene glycol

Abstract

A method is described to deliver mRNA to plant protoplasts using polyethylene glycol for transient expression. In order to optimize delivery and subsequent expression, we have examined a number of parameters that affect expression levels including the requirement for Mg2+ and Ca2+ ions, carrier nucleic acid, and the linearity of the dose-response. Delivery… (More)
DOI: 10.1007/BF00235304

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@article{Gallie1993IntroductionOM, title={Introduction of mRNA to plant protoplasts using polyethylene glycol}, author={Daniel R Gallie}, journal={Plant Cell Reports}, year={1993}, volume={13}, pages={119-122} }