Introduction of hepatitis delta virus into animal cell lines via cationic liposomes.

@article{Bichko1994IntroductionOH,
  title={Introduction of hepatitis delta virus into animal cell lines via cationic liposomes.},
  author={Vadim V. Bichko and Hans J{\"u}rgen Netter and John C. Taylor},
  journal={Journal of virology},
  year={1994},
  volume={68 8},
  pages={5247-52}
}
Cationic liposomes are known to facilitate efficient transfection of animal cells with DNA and even some viruses. As reported here, we have been able to use such a commercially available formulation (Lipofectamine) and introduce human hepatitis delta virus (HDV) into lines of cultured cells and demonstrate replication of the HDV genome both by immunofluorescence and by Northern (RNA) analysis. As much as 10% of the human hepatoma cell line Huh7 was transfected with HDV. Also transfected were… CONTINUE READING
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