Introduction of chloramphenicol resistance into the modified mouse mitochondrial genome: cloning of unstable sequences by passage through yeast.

@article{Bigger2000IntroductionOC,
  title={Introduction of chloramphenicol resistance into the modified mouse mitochondrial genome: cloning of unstable sequences by passage through yeast.},
  author={Brian W Bigger and Oleg E. Tolmachov and J M Collombet and Charles Coutelle},
  journal={Analytical biochemistry},
  year={2000},
  volume={277 2},
  pages={236-42}
}
Despite increasing awareness of the importance of the mitochondrial genome in human pathology, very few attempts have been made so far toward genetic engineering of mitochondrial DNA (mtDNA). One of the reasons for this slow progress is the difficulty of cloning mtDNA in Escherichia coli, a trait in common with repetitive or palindromic sequences, and some viral sequences. We have previously made a construct containing the entire mouse mitochondrial genome and a cDNA sequence coding for human… CONTINUE READING

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