Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.

@article{Mansour1990IntroductionOA,
  title={Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.},
  author={Suzanne L Mansour and Kirk Thomas and C X Deng and Mario R Capecchi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 19},
  pages={
          7688-92
        }
}
We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider spectrum of designed genomic alterations is now feasible. As a first application, we inserted a 5.4-kilobase cassette of nonhomologous DNA into the int-2 locus in mouse embryo-derived stem cells by gene targeting. The inserted DNA contained a lacZ gene positioned to create an in-frame fusion with the int… CONTINUE READING

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