Introduction and expression of the 400 kilobase precursor amyloid protein gene in transgenic mice

@article{Lamb1993IntroductionAE,
  title={Introduction and expression of the 400 kilobase precursor amyloid protein gene in transgenic mice},
  author={Bruce T. Lamb and Sangram S. Sisodia and Ann M. Lawler and Hilda H. Slunt and Cheryl A. Kitt and William G. Kearns and Peter L Pearson and Donald L. Price and John D. Gearhart},
  journal={Nature Genetics},
  year={1993},
  volume={5},
  pages={22-30}
}
Overexpression of the gene encoding the β–amyloid precursor protein (APP) may have a key role in the pathogenesis of both Alzheimer's disease (AD) and Down Syndrome (DS). We have therefore introduced a 650 kilobase (kb) yeast artificial chromosome (YAC) that contains the entire, unrearranged 400 kb human APP gene into mouse embryonic stem (ES) cells by lipid–mediated transfection. ES lines were generated that contain a stably integrated, unrearranged human APP gene. Moreover, we demonstrate… 
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