Introducing GUt low-density array (GULDA): a validated approach for qPCR-based intestinal microbial community analysis.
@article{Bergstrm2012IntroducingGL, title={Introducing GUt low-density array (GULDA): a validated approach for qPCR-based intestinal microbial community analysis.}, author={A. Bergstr{\"o}m and T. R. Licht and A. Wilcks and J. B. Andersen and L. R. Schmidt and H. Gr{\o}nlund and Louise K Vigsnaes and K. Michaelsen and M. Bahl}, journal={FEMS microbiology letters}, year={2012}, volume={337 1}, pages={ 38-47 } }
Alterations in the human gut microbiota caused, for example, by diet, functional foods, antibiotics, or occurring as a function of age are now known to be of relevance for host health. Therefore, there is a strong need for methods to detect such alterations in a rapid and comprehensive manner. In the present study, we developed and validated a high-throughput real-time quantitative PCR-based analysis platform, termed 'GUt Low-Density Array' (GULDA). The platform was designed for simultaneous… CONTINUE READING
54 Citations
Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa
- Biology, Medicine
- Molecular and Cellular Probes
- 2020
- 2
Associations between common intestinal parasites and bacteria in humans as revealed by qPCR
- Biology, Medicine
- European Journal of Clinical Microbiology & Infectious Diseases
- 2016
- 26
Impact of the gut microbiota on rodent models of human disease.
- Biology, Medicine
- World journal of gastroenterology
- 2014
- 50
- PDF
A combined metabolomic and phylogenetic study reveals putatively prebiotic effects of high molecular weight arabino-oligosaccharides when assessed by in vitro fermentation in bacterial communities derived from humans.
- Biology, Medicine
- Anaerobe
- 2014
- 19
- PDF
Microbial Enterotypes, Inferred by the Prevotella-to-Bacteroides Ratio, Remained Stable during a 6-Month Randomized Controlled Diet Intervention with the New Nordic Diet
- Biology, Medicine
- Applied and Environmental Microbiology
- 2013
- 85
- PDF
Quantifying Technical Confounders in Microbiome Studies.
- Biology, Medicine
- Cardiovascular research
- 2020
- 2
Establishment of Intestinal Microbiota during Early Life: a Longitudinal, Explorative Study of a Large Cohort of Danish Infants
- Biology, Medicine
- Applied and Environmental Microbiology
- 2014
- 247
- PDF
A Review of Applied Aspects of Dealing with Gut Microbiota Impact on Rodent Models.
- Medicine, Biology
- ILAR journal
- 2015
- 20
- PDF
Microbiotas from UC patients display altered metabolism and reduced ability of LAB to colonize mucus
- Biology, Medicine
- Scientific reports
- 2013
- 35
- PDF
Assessing non-digestible compounds in apple cultivars and their potential as modulators of obese faecal microbiota in vitro.
- Biology, Medicine
- Food chemistry
- 2014
- 26
- PDF
References
SHOWING 1-10 OF 55 REFERENCES
Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules
- Medicine, Biology
- Applied and Environmental Microbiology
- 2009
- 204
- PDF
Characterisation of intestinal bacteria in infant stools using real-time PCR and northern hybridisation analyses.
- Biology, Medicine
- FEMS microbiology ecology
- 2005
- 142
- PDF
Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces
- Biology, Medicine
- Applied and Environmental Microbiology
- 2004
- 588
- PDF
Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.
- Biology, Medicine
- Microbiological research
- 2008
- 151
Application of molecular biological methods for studying probiotics and the gut flora.
- Biology, Medicine
- The British journal of nutrition
- 2002
- 155
- PDF
Development of Bacteroides 16S rRNA Gene TaqMan-Based Real-Time PCR Assays for Estimation of Total, Human, and Bovine Fecal Pollution in Water
- Biology, Medicine
- Applied and Environmental Microbiology
- 2006
- 496
- PDF
Analysis of the intestinal microbiota of oligosaccharide fed mice exhibiting reduced resistance to Salmonella infection.
- Biology, Medicine
- Beneficial microbes
- 2010
- 29
Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA
- Biology, Medicine
- Applied and Environmental Microbiology
- 2002
- 676
- PDF
Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults
- Medicine, Biology
- PloS one
- 2010
- 1,618
- PDF
Improvement of phylum- and class-specific primers for real-time PCR quantification of bacterial taxa.
- Biology, Medicine
- Journal of microbiological methods
- 2011
- 241