Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.

@article{Ishida2012IntrinsicNA,
  title={Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.},
  author={M. Ishida and Hideaki Shimojo and Aki Hayashi and Rika Kawaguchi and Yasuko Ohtani and Koichi Uegaki and Yoshifumi Nishimura and Jun-Ichi Nakayama},
  journal={Molecular cell},
  year={2012},
  volume={47 2},
  pages={
          228-41
        }
}
Centromeric heterochromatin assembly in fission yeast requires the RNAi pathway. Chp1, a chromodomain (CD) protein, forms the Ago1-containing RNA-induced transcriptional silencing (RITS) complex and recruits siRNA-bound RITS to methylated histone H3 lysine 9 (H3K9me) via its CD. Here, we show that the CD of Chp1 (Chp1-CD) possesses unique nucleic acid-binding activities that are essential for heterochromatic gene silencing. Detailed electrophoretic-mobility shift analyses demonstrated that Chp1… CONTINUE READING
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