Intricate interactions within the ccd plasmid addiction system.

  title={Intricate interactions within the ccd plasmid addiction system.},
  author={Minh-Hoa Dao-Thi and Dani{\"e}l Charlier and Remy Loris and Dominique Maes and Joris Messens and Lode Wyns and Jan Backmann},
  journal={The Journal of biological chemistry},
  volume={277 5},
The ccd addiction system plays a crucial role in the stable maintenance of the Escherichia coli F plasmid. It codes for a stable toxin (CcdB) and a less stable antidote (CcdA). Both are expressed at low levels during normal cell growth. Upon plasmid loss, CcdB outlives CcdA and kills the cell by poisoning gyrase. The interactions between CcdB, CcdA, and its promoter DNA were analyzed. In solution, the CcdA-CcdB interaction is complex, leading to various complexes with different stoichiometry… CONTINUE READING