Intrasarcomere [Ca2+] gradients and their spatio-temporal relation to Ca2+ sparks in rat cardiomyocytes.


1. Line-scan analyses of spontaneous Ca2+ sparks, non-propagating local rises in Ca2+ concentration, and the early phase of Ca2+ transients in cardiomyocytes were performed with a rapid-scanning laser confocal microscope (Nikon RCM8000) and fluo-3. 2. On electrical stimulation, points at which rise in Ca2+ began earliest were observed at regular spacings of… (More)


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