Intrapleural adenoviral delivery of human plasminogen activator inhibitor-1 exacerbates tetracycline-induced pleural injury in rabbits.

@article{Karandashova2013IntrapleuralAD,
  title={Intrapleural adenoviral delivery of human plasminogen activator inhibitor-1 exacerbates tetracycline-induced pleural injury in rabbits.},
  author={Sophia Karandashova and Galina Florova and Ali O. Azghani and Andrey A. Komissarov and Kathy Koenig and Torry A. Tucker and Timothy Craig Allen and Kris Stewart and Amy R. Tvinnereim and Steven Idell},
  journal={American journal of respiratory cell and molecular biology},
  year={2013},
  volume={48 1},
  pages={44-52}
}
Elevated concentrations of plasminogen activator inhibitor-1 (PAI-1) are associated with pleural injury, but its effects on pleural organization remain unclear. A method of adenovirus-mediated delivery of genes of interest (expressed under a cytomegalovirus promoter) to rabbit pleura was developed and used with lacZ and human (h) PAI-1. Histology, β-galactosidase staining, Western blotting, enzymatic and immunohistochemical analyses of pleural fluids (PFs), lavages, and pleural mesothelial… CONTINUE READING