Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates.

@article{Yu2015IntrapersonalMO,
  title={Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates.},
  author={Wen-Liang Yu and Mei-Feng Lee and Ming-Chung Chang and Y N Chuang},
  journal={Journal of global antimicrobial resistance},
  year={2015},
  volume={3 2},
  pages={
          137-141
        }
}
Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE). However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. A previously unrelated liver abscess K. pneumoniae hypermucoviscous isolate was used as a control. PCR, DNA cloning and sequencing for the plasmid-borne rmpA and rmpA2 genes and the… CONTINUE READING