Intranasal immunization of a DNA vaccine with IL-12- and granulocyte-macrophage colony-stimulating factor (GM-CSF)-expressing plasmids in liposomes induces strong mucosal and cell-mediated immune responses against HIV-1 antigens.

@article{Okada1997IntranasalIO,
  title={Intranasal immunization of a DNA vaccine with IL-12- and granulocyte-macrophage colony-stimulating factor (GM-CSF)-expressing plasmids in liposomes induces strong mucosal and cell-mediated immune responses against HIV-1 antigens.},
  author={Eriko Okada and Shin Sasaki and Naoto Ishii and Ichiro Aoki and Tadanobu Yasuda and Kusuki Nishioka and Jun Fukushima and Jun-Ichi Miyazaki and Britta E Wahren and Kenji Okuda},
  journal={Journal of immunology},
  year={1997},
  volume={159 7},
  pages={3638-47}
}
A DNA vaccine constructed with the CMV promoter conjugated to env gp160 and rev genes has been shown to induce an effective Th1-type immune response when inoculated via an intramuscular route. In the present study, we obtained high levels of both humoral and cell-mediated immune activity by intranasal administration of this DNA vaccine. The production of mucosal IgA Ab in feces and vaginal fluid was stimulated significantly by intranasal DNA administration. This route of administration resulted… CONTINUE READING