Intranasal administration of plasmid DNA nanoparticles yields successful transfection and expression of a reporter protein in rat brain

@article{Harmon2014IntranasalAO,
  title={Intranasal administration of plasmid DNA nanoparticles yields successful transfection and expression of a reporter protein in rat brain},
  author={Brendan Harmon and Amirah E-E Aly and Linas Padegimas and Ozge Sesenoglu-Laird and Mark J. Cooper and Barbara L. Waszczak},
  journal={Gene Therapy},
  year={2014},
  volume={21},
  pages={514-521}
}
Viral vectors are a commonly used method for gene therapy because of their highly efficient transduction of cells. However, many vectors have a small genetic capacity, and their potential for immunogenicity can limit their usefulness. Moreover, for disorders of the central nervous system (CNS), the need for invasive surgical delivery of viruses to the brain also detracts from their clinical applicability. Here, we show that intranasal delivery of unimolecularly compacted DNA nanoparticles (DNA… Expand
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