Intramolecular electron transfer in sulfite-oxidizing enzymes: probing the role of aromatic amino acids

@article{Rajapakshe2011IntramolecularET,
  title={Intramolecular electron transfer in sulfite-oxidizing enzymes: probing the role of aromatic amino acids},
  author={Asha Rajapakshe and Kimberly T. Meyers and Robert E. Berry and Gordon Tollin and John H. Enemark},
  journal={JBIC Journal of Biological Inorganic Chemistry},
  year={2011},
  volume={17},
  pages={345-352}
}
Sulfite oxidase (SO) is a molybdoheme enzyme that is important in sulfur catabolism, and mutations in the active site region are known to cause SO deficiency disorder in humans. This investigation probes the effects that mutating aromatic residues (Y273, W338, and H337) in the molybdenum-containing domain of human SO have on both the intramolecular electron transfer (IET) rate between the molybdenum and iron centers using laser flash photolysis and on catalytic turnover via steady-state kinetic… CONTINUE READING