Intramolecular cyclizations of polyketide biosynthesis: mining for a "Diels-Alderase"?

Despite the large number of naturally occurring metabolites existing for which enzymatic Diels-Alder reactions have been proposed as a key biosynthetic step, the actual number of enzymes thus far identified for these transformations is incredibly low. Even for those few enzymes identified, there is currently little biochemical or mechanistic evidence to… CONTINUE READING