Intramembranous cytochemistry: a new morphological technique for studying cholesterol in the astrocyte plasma membrane of ischemic brain cells.

The distribution of cholesterol in the astrocytic plasma membranes of normal and ischemic caudate nucleus in the cat was investigated with the polyene antibiotic, filipin, a morphological probe for membrane cholesterol domains in freeze-fracture tissue replicas. After filipin incubation of prefixed vibratome slices, filipin-cholesterol complexes appeared as… CONTINUE READING