Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa.

@article{Li2003IntracytoplasmicSI,
  title={Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa.},
  author={Guanghao Li and George E. Seidel and Edward L. Squires},
  journal={Theriogenology},
  year={2003},
  volume={59 5-6},
  pages={
          1143-55
        }
}
Five experiments were designed to study the fertilizability and development of bovine oocytes fertilized by intracytoplasmic sperm injection (ICSI) with stallion spermatozoa. Experiment 1 determined the time required for pronuclear formation after ICSI. Equine sperm head decondensation began 3 h after ICSI; 42% were decondensed 6 h after ICSI. Male pronuclei (MPN) began to form 12 h after ICSI. Female pronuclei (FPN), however, formed as early as 6 h after ICSI. In Experiment 2, ionomycin… CONTINUE READING
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