Intracytoplasmic sperm injection in the rat.

  title={Intracytoplasmic sperm injection in the rat.},
  author={Dmitri I Dozortsev and T Wakaiama and Alexandre N. Ermilov and Ryuzo Yanagimachi},
  volume={6 2},
We applied intracytoplasmic sperm injection (ICSI) to the rat comparing three different sperm injection techniques: conventional setup with a sharp needle bearing a spike (method 1), combination of partial zona dissection (PZD) needle and blunt pipette (method 2) and piezo-injection using a blunt pipette (method 3). We also investigated the timing of sperm pronuclear formation after injection. Survival rates after injection were 8%, 24% and 71% for the methods 1, 2 and 3, respectively. All… CONTINUE READING


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