Intracytoplasmic sperm injection in the mouse.

  title={Intracytoplasmic sperm injection in the mouse.},
  author={R Ron-el and J Liu and Zolt{\'a}n Nagy and Hubert Joris and Etienne van den Abbeel and Andr{\'e} C. van Steirteghem},
  journal={Human reproduction},
  volume={10 11},
Intracytoplasmic sperm injection (ICSI) into mouse oocytes involves a very low survival rate. This study was designed to determine why ICSI frequently fails in mice. Metaphase II oocytes were obtained from superovulated 4-6 week old F1 hybrid mice. Spermatozoa were retrieved from the epididymis of 12-14 week old F1 hybrid mice. The spiked microinjection pipette used to inject a spermatozoon into the ooplasm had outer and inner diameters of 10 and 8 microns respectively. The oocytes used in the… CONTINUE READING
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