Intracytoplasmic sperm injection in the mouse.

@article{Ronel1995IntracytoplasmicSI,
  title={Intracytoplasmic sperm injection in the mouse.},
  author={R Ron-el and J Liu and Zolt{\'a}n Nagy and Hubert Joris and Etienne van den Abbeel and Andr{\'e} C. van Steirteghem},
  journal={Human reproduction},
  year={1995},
  volume={10 11},
  pages={2831-4}
}
Intracytoplasmic sperm injection (ICSI) into mouse oocytes involves a very low survival rate. This study was designed to determine why ICSI frequently fails in mice. Metaphase II oocytes were obtained from superovulated 4-6 week old F1 hybrid mice. Spermatozoa were retrieved from the epididymis of 12-14 week old F1 hybrid mice. The spiked microinjection pipette used to inject a spermatozoon into the ooplasm had outer and inner diameters of 10 and 8 microns respectively. The oocytes used in the… CONTINUE READING
Highly Influential
This paper has highly influenced 38 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
272 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 272 extracted citations

Similar Papers

Loading similar papers…