Intracellularly trapped pH indicators.

  title={Intracellularly trapped pH indicators.},
  author={James A Thomas},
  journal={Society of General Physiologists series},
  • James A Thomas
  • Published 1986 in Society of General Physiologists series
The trapped indicator technique has several advantages for monitoring intracellular pH. It can be performed with equipment available in most laboratories, using either fluorescence or absorbance measurements. It is nondestructive to cells, and fluorescein dyes seem to have little effect on cell metabolism (however, see Spray et al., 1984). Continuous real-time monitoring of intracellular pH is possible, and the method can detect changes of 0.01 pH. It is sensitive enough for studies with… CONTINUE READING

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