Intracellular trafficking and membrane targeting mechanisms of the human reduced folate carrier in Mammalian epithelial cells.

@article{Marchant2002IntracellularTA,
  title={Intracellular trafficking and membrane targeting mechanisms of the human reduced folate carrier in Mammalian epithelial cells.},
  author={Jonathan S. Marchant and V. Sankara Subramanian and Ian Parker and Hamid Said},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 36},
  pages={33325-33}
}
The major pathway for cellular uptake of the water-soluble vitamin folic acid in mammalian cells is via a plasma membrane protein known as the reduced folate carrier (RFC). The molecular determinants that dictate plasma membrane expression of RFC as well as the cellular mechanisms that deliver RFC to the cell surface remain poorly defined. Therefore, we designed a series of fusion proteins of the human RFC (hRFC) with green fluorescent protein to image the targeting and trafficking dynamics of… CONTINUE READING