Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.

@article{Rosazza2013IntracellularTO,
  title={Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.},
  author={Christelle Rosazza and Annette Buntz and Thorsten Riess and Dominik W{\"o}ll and Andreas Zumbusch and Marie-Pierre Rols},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2013},
  volume={21 12},
  pages={
          2217-26
        }
}
Electroporation is a physical method of transferring molecules into cells and tissues. It takes advantage of the transient permeabilization of the cell membrane induced by electric field pulses, which gives hydrophilic molecules access to the cytoplasm. This method offers high transfer efficiency for small molecules that freely diffuse through electrically permeabilized membranes. Larger molecules, such as plasmid DNA, face several barriers (plasma membrane, cytoplasmic crowding, and nuclear… CONTINUE READING
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