Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices.

@article{Aghajanian1989IntracellularSI,
  title={Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices.},
  author={George K. Aghajanian and Keith G. Rasmussen},
  journal={Synapse},
  year={1989},
  volume={3 4},
  pages={331-8}
}
In general, it has been difficult to preserve electrophysiologically viable motoneurons in brain slices from adult mammals. The present study describes a new method for obtaining viable motoneurons in the facial nucleus of adult rat brain slices. The essence of the method was to use a modified artificial cerebrospinal fluid (ACSF) in which NaCl was replaced initially by sucrose; the modified ACSF was used during 1) preparation and 2) a 1 hr recovery period. The rationale for the modification is… CONTINUE READING
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