Intracellular stability of diphtheria toxin fragment A in the presence and absence of anti-fragment A antibody.

@article{Yamaizumi1982IntracellularSO,
  title={Intracellular stability of diphtheria toxin fragment A in the presence and absence of anti-fragment A antibody.},
  author={Masaru Yamaizumi and Tsuyoshi Uchida and Kimiko Takamatsu and Yukihiko Okada},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 2},
  pages={
          461-5
        }
}
The erythrocyte ghost function method was used to introduce 125I-labeled diphtheria toxin, its fragments A and B, and two labeled crossreacting material (CRM), mutant proteins CRM176 and CRM197, into cultured mouse cells. Fragment A was relatively stable in mouse cytoplasm at 37 degrees C and at least 80% was recovered from cells after 24 hr of incubation. In contrast, wild-type fragment B and A fragments from CRM176 and CRM197 were unstable and were degraded with half-lives of about 2.5 hr… CONTINUE READING

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