Intracellular retention of interleukin-6 abrogates signaling.

  title={Intracellular retention of interleukin-6 abrogates signaling.},
  author={Stefan Rose-John and Heidi Schooltink and Hildegard Schmitz-Van de Leur and J{\"u}rgen M{\"u}llberg and Peter Claus Heinrich and Lutz Graeve},
  journal={The Journal of biological chemistry},
  volume={268 29},
Three forms of interleukin-6 (IL-6) have been constructed and stably transfected into human hepatoma cells (HepG2). Wild type IL-6 containing a signal peptide was rapidly secreted as a biologically active protein. IL-6 lacking the signal peptide accumulated within the cytoplasm of transfected cells. Surprisingly, IL-6 carrying a COOH-terminal extension of the amino acids Lys-Asp-Glu-Leu (KDEL) was not completely retained in the endoplasmic reticulum (ER). Complete retention in the ER was… CONTINUE READING