Intracellular protein production in Trichoderma reesei (Hypocrea jecorina) with hydrophobin fusion technology.

@article{Mustalahti2013IntracellularPP,
  title={Intracellular protein production in Trichoderma reesei (Hypocrea jecorina) with hydrophobin fusion technology.},
  author={Eero Mustalahti and Markku Saloheimo and Jussi Joonas Joensuu},
  journal={New biotechnology},
  year={2013},
  volume={30 2},
  pages={262-8}
}
Insufficient accumulation and the lack of efficient purification methods are the two major bottlenecks hindering the recombinant production of many proteins. Alternative production schemes are urgently needed for proteins that remain challenging to express and purify with conventional techniques. We have found that hydrophobin fusions targeted to endoplasmic reticulum (ER) can enhance the expression of target proteins simultaneously providing means for straightforward purification. Here we show… CONTINUE READING

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