Intracellular potentials in rabbit proximal tubules perfused in vitro.

  title={Intracellular potentials in rabbit proximal tubules perfused in vitro.},
  author={Bruno Biagi and Toshiro Kubota and Morgan E. H. Sohtell and Gerhard Giebisch},
  journal={The American journal of physiology},
  volume={240 3},
Conventional microelectrodes were used to measure the basolateral membrane potential (VBL) in isolated perfused superficial proximal convoluted (sPCT) and superficial proximal straight (sPST) tubules of the rabbit kidney. Stable recordings for periods up to 2 h can be obtained. The mean +/- SE (n = number of cells) values of VBL were sPCT = -51.0 +/- 1.63 (24) and sPST = -47.0 +/- 0.97 (94) mV. Inhibitors of active transport, ouabain (10(-5) M) and low bath potassium (0.1 mM), caused a… CONTINUE READING

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