Intracellular particle dissolution in macrophages isolated from the lung of the Fischer (F-344) rat.

@article{Harper1994IntracellularPD,
  title={Intracellular particle dissolution in macrophages isolated from the lung of the Fischer (F-344) rat.},
  author={Robert Anthony Harper and Charles A. Stirling and K. M. Stuart Townsend and Wolfgang G Kreyling and Graham L Patrick},
  journal={Experimental lung research},
  year={1994},
  volume={20 2},
  pages={143-56}
}
Alveolar macrophages were removed from male F-344 rats by bronchoalveolar lavage and maintained in vitro for 14 days. Over this period the macrophages remained viable as judged by propidium iodide exclusion, lysosomal uptake of acridine orange, and phagocytosis of 1.75-microns latex beads. After 7 and 14 days of culture the cells contained lipid droplets. The macrophages were shown to ingest and dissolve monodisperse 57Co3O4 particles, which were relatively insoluble in extracellular medium… CONTINUE READING