Intracellular pH regulation in neurons from chemosensitive and nonchemosensitive regions of Helix aspersa.

@article{Goldstein2000IntracellularPR,
  title={Intracellular pH regulation in neurons from chemosensitive and nonchemosensitive regions of Helix aspersa.},
  author={J I Goldstein and Jie-Men Mok and Cynthia M. Simon and James C Leiter},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  year={2000},
  volume={279 2},
  pages={
          R414-23
        }
}
  • J. I. Goldstein, J. Mok, +1 author J. Leiter
  • Published 1 August 2000
  • Biology
  • American journal of physiology. Regulatory, integrative and comparative physiology
We used 2',7'-bis(carboxyethyl)-5(6)-carboxyflourescein (BCECF), a pH-sensitive fluorescent dye, to study intracellular pH (pH(i)) regulation in neurons in CO(2) chemoreceptor and nonchemoreceptor regions in the pulmonate, terrestrial snail, Helix aspersa. We studied pH(i) during hypercapnic acidosis, after ammonia prepulse, and during isohydric hypercapnia. In all treatment conditions, pH(i) fell to similar levels in chemoreceptor and nonchemoreceptor regions. However, pH(i) recovery was… 
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P pH( i) regulation during acidosis is more effective in the hypoglossal nucleus in younger animals, possibly as a requirement of development, but in older juvenile animals (older than postnatal day 11), pH(i) regulation is relatively poor in chemosensitive (RTN) and nonchemosensitive nuclei (hypoglossAL nucleus).
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PH(i) recovery is mediated by different pH-regulating transporters in neurons from different chemosensitive regions, but recovery is suppressed by hypercapnia in all of the neurons.
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There is evidence of Na+-HCO3- cotransport and Na+/H+ exchange in glia in the RTN and NTS, but the distribution of glia and the distributionof these pH-regulatory functions are not identical in the NTS and RTN.
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In this work, attempts to clamp intracellular pH in the presence of hypercapnic acidosis (HA) in neurons from the locus coeruleus and nucleus of the solitary tract in neonatal rat brainstem slices are made.
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The results of the model indicate that multiple channels contribute to CO(2) chemosensitivity, but the primary sensor is probably I(KA), and pH(i) may be a sufficient chemosensory stimulus, but it may not be a necessary stimulus: either pH( i) or extracellular pH can be an effective stimuli if chemOSensory neurons express appropriate pH-sensitive channels.
CO2 chemoreception in cardiorespiratory control.
  • R. Putnam
  • Biology
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  • 2010
TLDR
Fully describing the chemosensitive signaling pathways in brain stem neurons will offer new targets for therapies to alter the strength of central chemosensitivity and will yield new insights into the reason why there are multiple central chemoreceptive sites.
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