Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method

@article{Johnson1981IntracellularPO,
  title={Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method},
  author={Carl Hirschie Johnson and David Epel},
  journal={The Journal of Cell Biology},
  year={1981},
  volume={89},
  pages={284 - 291}
}
Intracellular pH (pH1) of sea urchin eggs and embryos was determined using DMO (5,5-dimethyl-2,4-oxazolidinedione). By this method, the pH1 of Lytechinus pictus eggs increased after fertilization from 6.86 to 7.27, and this higher pHi was maintained thereafter, as has been previously observed with pH microelectrodes. The same general result was obtained with the eggs of Strongylocentrotus purpuratus, in contrast to previous estimates of the pH of egg homogenates from this species, which had… CONTINUE READING