Intracellular pH of halobacteria can be determined by the fluorescent dye 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein.

@article{Tsujimoto1988IntracellularPO,
  title={Intracellular pH of halobacteria can be determined by the fluorescent dye 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein.},
  author={K Tsujimoto and M Semadeni and M E Huflejt and Lester Packer},
  journal={Biochemical and biophysical research communications},
  year={1988},
  volume={155 1},
  pages={123-9}
}
Determination of the internal pH of halobacterial cells grown in 4M salt solution has proven to be a difficult problem. We now report the steady state cytosolic pH of Halobacterium halobium S-9 to be 7.2. Intracellular pH was determined after the cells were loaded with the membrane permeable precursor of the pH sensitive dye 2', 7'-bis-(carboxyethyl)-5(6)-carboxyfluorescein-acetyxymethyl ester - (BCECF/AM). In order to minimize light-scattering in the measurement of the fluorescence, a thin… CONTINUE READING

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