Intracellular pH-measurements in rat duodenal mucosa in vitro using confocal laserscan microscopy.

@article{Weinlich1997IntracellularPI,
  title={Intracellular pH-measurements in rat duodenal mucosa in vitro using confocal laserscan microscopy.},
  author={Michael Weinlich and U. Heydasch and Michael Starlinger and Rolf K. H. Kinne},
  journal={Zeitschrift fur Gastroenterologie},
  year={1997},
  volume={35 4},
  pages={263-70}
}
An improved technique was developed to measure intracellular pH-changes in in vitro duodenal mucosa. A confocal laserscan microscope was equipped with a second laser to permit dual wavelength excitation measurements employing BCECF (2'7'-bis-2-carboxyethyl-5-(and-6)carboxyfluorescein), a pH-sensitive fluorescent dye. Intact rat duodenal epithelium was mounted in a microperfusion chamber and loaded with BCECF via submucosal injection. Viability of the epithelial cells could be directly monitored… CONTINUE READING

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