Intracellular pH in isolated Necturus duodenal mucosa exposed to luminal acid.

@article{Paimela1992IntracellularPI,
  title={Intracellular pH in isolated Necturus duodenal mucosa exposed to luminal acid.},
  author={Hannu M Paimela and Tuula Kiviluoto and H. Mustonen and Eero O. Kivilaakso},
  journal={Gastroenterology},
  year={1992},
  volume={102 3},
  pages={862-7}
}
Regulation of intracellular pH (pHi) and its maintenance within physiological ranges during exposure to luminal acid was studied in isolated Necturus duodenal mucosa using liquid sensor microelectrodes. Exposure of the mucosa to luminal pH 2.7 caused significant intraepithelial acidification. Subsequent removal of HCO3-/CO2 (HEPES/O2 substitution) from the serosal perfusate caused a further decrease of pHi. Blocking of HCO3- transport across the basolateral cell membrane by addition of 4… CONTINUE READING