Intracellular location of thymidylate synthase and its state of phosphorylation.

  title={Intracellular location of thymidylate synthase and its state of phosphorylation.},
  author={William A. Samsonoff and James Reston and Mary Mckee and Brian J O'connor and John Galivan and Gladys F. Maley and Frank Maley},
  journal={The Journal of biological chemistry},
  volume={272 20},
Thymidylate synthase (TS), an enzyme that is essential for DNA synthesis, was found to be associated mainly with the nucleolar region of H35 rat hepatoma cells, as determined both by immunogold electron microscopy and by autoradiography. In the latter case, the location of TS was established through the use of [6-3H]5-fluorodeoxyuridine, which forms a tight ternary complex of TS with 5-fluorodeoxyuridylate (FdUMP) and 5, 10-methylenetetrahydrofolylpolyglutamate within the cell. However, with… CONTINUE READING


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