Intracellular location of the Saccharomyces cerevisiae CDC6 gene product.

  title={Intracellular location of the Saccharomyces cerevisiae CDC6 gene product.},
  author={Ambrose Y. Jong and Marian G. Young and George C. Chen and Shuang quan Zhang and C. Chan},
  journal={DNA and cell biology},
  volume={15 10},
The CDC6 gene product from Saccharomyces cerevisiae is required for transition from late G1 to S phase of the cell cycle. We have investigated the subcellular localization of the CDC6 protein in yeast to explore where Cdc6p exerts its gene function (s). Using affinity-purified sera we localized Cdc6p to the cytoplasm and the nuclear matrix by both subcellular fractionation and indirect immunofluorescence microscopy. The nuclear localization was confirmed to be in the nuclear scaffold by the low… CONTINUE READING

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