Intracellular location of acetyltransferase in the remodeling pathway of PAF biosynthesis in undifferentiated human leukemic cells (HL-60).

Abstract

In order to determine the site of PAF synthesis via the remodeling pathway in undifferentiated HL-60 cells, a subcellular fractionation procedure (Ribbes et al., 1985) was adapted to separate various membrane fractions and cellular organelles of HL-60 cells across a Percoll gradient. The profile of alkyllyso-GPC:acetyl-CoA acetyltransferase activity closely… (More)

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