Intracellular localization and association of MHC class I with porcine invariant chain.

  title={Intracellular localization and association of MHC class I with porcine invariant chain.},
  author={F. Z. Xu and S G Wu and W. Y. Yu},
  journal={Genetics and molecular research : GMR},
  volume={12 1},
The objective was to investigate the intracellular localization and association of pig major histocompatibility complex (MHC) class I subunits with invariant chain (Ii). Pig MHC class I subunit cDNAs were cloned by RT-PCR and eukaryotic expression plasmids of α and β2m were constructed with fusions to red or enhanced green fluorescent protein (pDsRed2-N1-α, pEGFP-N1-α, pDsRed2-N1-β2m, and pEGFP-N1-β2m). A pig Ii mutant with a deleted CLIP region (DCLIP-Ii) was constructed by overlap extension… 

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Both invariant chain isoforms Ii31 and Ii41 promote class II antigen presentation.

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The CLIP region of invariant chain plays a critical role in regulating major histocompatibility complex class II folding, transport, and peptide occupancy

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An unstable beta 2-microglobulin: major histocompatibility complex class I heavy chain intermediate dissociates from calnexin and then is stabilized by binding peptide

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